Crystal Structures of Fumarate Hydratases from Leishmania major in a Complex with Inhibitor 2-Thiomalate
نویسندگان
چکیده
منابع مشابه
Crystal structure of the Leishmania major peroxidase-cytochrome c complex.
The causative agent of leishmaniasis is the protozoan parasite Leishmania major. Part of the host protective mechanism is the production of reactive oxygen species including hydrogen peroxide. In response, L. major produces a peroxidase, L. major peroxidase (LmP), that helps to protect the parasite from oxidative stress. LmP is a heme peroxidase that catalyzes the peroxidation of mitochondrial ...
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A series of aurones was analyzed for the ability to inhibit respiratory functions of mitochondria of Leishmania parasites. The aim of this study was to find a rational explanation for the activity of certain aurones and auronols as novel antiprotozoal compounds of plant origin. In a cell-free assay mitochondrial fumarate reductase from L. donovani was inhibited in a concentration-dependent mann...
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Fumarate hydratases (FHs) are essential metabolic enzymes grouped into two classes. Here, we present the crystal structure of a class I FH, the cytosolic FH from Leishmania major, which reveals a previously undiscovered protein fold that coordinates a catalytically essential [4Fe-4S] cluster. Our 2.05 Å resolution data further reveal a dimeric architecture for this FH that resembles a heart, wi...
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The enzyme adenine phosphoribosyltransferase (APRT) functions to salvage adenine by converting it to adenosine-5-monophosphate (AMP). APRT deficiency in humans is a well characterized inborn error of metabolism, and APRT may contribute to the indispensable nutritional role of purine salvage in protozoan parasites, all of which lack de novo purine biosynthesis. We determined crystal structures f...
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Introduction: Cutaneous leishmaniasis (CL) is a widespread disease that is epidemic in Iran, too. Photodynamic therapy (PDT) is an attractive modality to treat cancer and hyper proliferative diseases based on the use of a photosensitizer in the presence of oxygen and proper wavelength of light. In consideration of lesion location, lack of systemic involvement and inefficiency of current treatme...
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ژورنال
عنوان ژورنال: ACS Chemical Biology
سال: 2019
ISSN: 1554-8929,1554-8937
DOI: 10.1021/acschembio.8b00972